The developed assay revealed to be sufficiently stable and robust to assess an activating EC 50-value. Instead of using PERK’s natural substrate proteins, eIF2α and NRF2, we applied SMAD3 as phosphorylation-accepting protein and successfully detected cell-free PERK activation and inhibition by selected modulators (e.g., calcineurin-B, GSK2606414). By applying the catalytic domain of recombinant human PERK, we initially determined ideal conditions of the kinase assay reaction, including parameters such as optimal kinase concentration, temperature, and reaction time. Our study aimed at the development of a cell-free screening assay enabling the detection of novel direct PERK activators. So far, a shortage of available direct PERK activators has been limiting the progresses in this field. Take note that the app just provides the info and cannot help you make any modifications.Īll in all, SYSInfo Monitor is a straightforward program that can gather and display details about your computer without too much hassle.The activation of the unfolded protein response, particularly via the PERK pathway, has been suggested as a promising therapeutic approach in tauopathies, a group of neurodegenerative disorders characterized by the abnormal phosphorylation and aggregation of tau protein. Other information that you can learn about your system via this tool includes printers, motherboard, BIOS, network, audio device and the operating system. For example, the app displays the total, used and available RAM and based on the numbers, you may want to determine what is hogging it. Despite the name, the app does not highlight various problems with the aforementioned elements, but rather it shows different aspects that can help advanced users investigate a potential issue further. The utility comes with a system diagnostic feature that targets the CPU, RAM and storage. The data can be copied to clipboard or saved as TXT or CSV. For instance, for the CPU, the app displays the full name, device ID, manufacturer, current clock speed, caption, number of cores and the corresponding temperatures, number of logical processors, architectures, address width and process type. The utility provides detailed information about the CPU, graphic card and storage media.
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